Just to add another reference in here since it was discussed about how hard it is to define MLSS
https://physoc.onlinelibrary.wiley.com/doi/full/10.14814/phy2.14098
(basically talks about why critical power is a better estimate than MLSS)
Before I saw that one I was interested in trying one out since I haven’t been having great results estimating MLSS through lactate testing. The 3 min continuous test is pretty easy to execute… if you are motivated. I think that NSCYD uses this as part of their testing protocol, which it seems that the NSCYD test is more about CP and how it fits in the mader and heck model to estimate LT1 and remaining power duration curve.
Here’s my charts from that effort: all way, way, above what I was getting for lactate measurements, probably because it takes close to 30 minutes before getting to a steady state as you had mentioned in your last response. CP from this effort would be 288, that is the 30s avg at 3 minutes, I kept going to the top of the hill, so the total duration was more like 5 and a half minutes since I had no idea what the duration was, and in the last 2 minutes the power dropped down to about 270 watts. When doing some of those old over under workouts that used to be a part of SSBII, it was clear though that even if that FTP was higher than what I could sustain for an hour, the over/unders were above/below that specific point.