Trainer resistance suddenly harder

Yes, retest.

The timeline is a bit confusing, but ANY TIME you change ANY equipment as part of a Virtual Power setup, you must retest. You did it properly with the tire change.

But I don’t see that you retested after swapping to the rollers. Without a dedicated power meter, you can’t just swap between trainers or rollers and expect the VP numbers to align. They just don’t work that way.

So a test in each device is necessary. And you can’t likely use the same FTP on both devices, unless you get a real power meter.